PDLs22 재조합 단백질의 합성과 평가
Synthesis and evaluation of PDLs22 recombinant protein
이경연, 이유진, 김흥중, 최용석, 박주철, 배현숙, 조광희, 장현선,
소속 상세정보
이경연 ( Lee Kyoung-Yeon ) - 소속확인중
이유진 ( Lee Eu-Gene ) - 소속확인중
김흥중 ( Kim Heung-Jung ) - 소속확인중
최용석 ( Choi Youn-Suk ) - 경희대학교 치과대학 구강악안면방사선과학교실
박주철 ( Park Joo-Cheol ) - 소속확인중
배현숙 ( Bae Hyun-Sook ) - 소속확인중
조광희 ( Cho Kwang-Hee ) - 소속확인중
장현선 ( Jang Hyun-Seon ) - 소속확인중
KMID : 0363020070370010035
Abstract
Periodontal ligament (PDL) is the connective tissue located between the tooth root and alveolar bone. In a previous study, PDLs22 was isolated as a PDL-specific gene by using subtractive hybrid-ization between cultured PDL fibroblasts and gingival fibroblasts. It was also suggested that PDLs22 plays important roles in the development, differentiation and maintenance of periodontal tissues. However, little is known about functional study of PDLs22 using recombinant protein in PDL fibroblast differentiation and periodontium formation. In this study, in order to produce the PDLs22 recombinat protein, PDLs22 expression vector were constructed and expressed its protein in various host cell and temperature conditions. The results were as follows: 1. PDLs22 protein was not strongly expressed In the induction system using pRSET-PDLs22 construct. 2. When the BL21(DE3) pLysS was used as a expression host, PDLS22 protein was strongly ex-pressed in the induction system using pHCEIIBNd-PDLs22 construct. 3. The PDLs22 protein was recognized at a molecular weight of 28 kDa in western blots. 4. Almost of the expressed PDLs22 protein was not soluble and observed like as inclusion body. 5. The protein solubility was not improved after modification of induction time and temperature during PDLs22 protein production. In this study, the system for the PDLs22 protein production was connstructed. However, the re-results suggest that further studies will be needed to produce the considerable amount of PDLs22 re-combinat protein, which can use for the periodontal regeneration.
키워드
PDLs22;recombinant protein;solubility;expression;UNCL
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